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  Table of Contents  
ORIGINAL ARTICLE
Year : 2020  |  Volume : 11  |  Issue : 5  |  Page : 738-746  

Clinical profile of overgrowth syndromes consistent with PROS (PIK3CA-related overgrowth syndromes)—A case series


1 Department of Dermatology, Venereology and Leprosy, Christian Medical College, Vellore, Tamil Nadu, India
2 Department of Radiodiagnosis, Christian Medical College, Vellore, Tamil Nadu, India
3 Department of Haematology, Christian Medical College, Vellore, Tamil Nadu, India

Date of Submission12-Oct-2019
Date of Decision07-Jan-2020
Date of Acceptance26-Feb-2020
Date of Web Publication19-Sep-2020

Correspondence Address:
Renu George
Department of Dermatology, Christian Medical College, Vellore - 632 004, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/idoj.IDOJ_520_19

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   Abstract 


Context: PIK3CA-related overgrowth syndrome (PROS) is characterized by focal and disproportionate growth of acral body structures in a mosaic pattern with varied phenotypes. Clinical diagnostic criteria are available and testing of the mutation is recommended for diagnosis. Cutaneous features described in these conditions include epidermal nevi and vascular malformations which form part of the diagnostic criteria. Aims: To detail the clinical profile of patients with presumptive PROS. Settings and Design: We conducted a retrospective study of 15 patients with focal overgrowth of the extremities or macrocephaly who presented to the department of dermatology at a tertiary care hospital in South India. Subjects and Methods: Data were collected through electronic medical records from July 2012 to April 2018 over 70 months. The criterion proposed by Keppler-Noreuil et al. was used for classifying them as presumptive PROS in the absence of genetic studies. Statistical Analysis Used: Descriptive analysis. Results: There were nine males and six females; mean age of 12.10 years (range: 8 months to 73 years) with clinical features consistent with PROS. There was a higher frequency of vascular malformations (9/15, 60%) and of epidermal nevi (7/15, 46.6%) than that reported in the literature. Unusual features included focal acrochordons, blaschkoid hypopigmentation and linear papillomatous growths in the oral mucosa. Conclusions: This study provides data on the clinical features of patients with PROS from the Indian subcontinent. In resource-poor settings, clinical criteria may be adequate for diagnosis due to restricted accessibility of technically challenging diagnostic tests.

Keywords: Cutaneous, overgrowth, PIK3CA, PIK3CA-related overgrowth syndrome


How to cite this article:
Mathew L, George R, Sudhakar S, Keshava SN, Fouzia N A. Clinical profile of overgrowth syndromes consistent with PROS (PIK3CA-related overgrowth syndromes)—A case series. Indian Dermatol Online J 2020;11:738-46

How to cite this URL:
Mathew L, George R, Sudhakar S, Keshava SN, Fouzia N A. Clinical profile of overgrowth syndromes consistent with PROS (PIK3CA-related overgrowth syndromes)—A case series. Indian Dermatol Online J [serial online] 2020 [cited 2020 Oct 30];11:738-46. Available from: https://www.idoj.in/text.asp?2020/11/5/738/295582




   Introduction Top


Focal overgrowth syndromes present with localized growth of the extremities, trunk, head, or the brain, in isolation or in various combinations in a mosaic pattern with or without epidermal and vascular nevi and variable patterns of acral bony abnormalities. With the detection of the causative mutation, they are better referred to as PIK3CA gene-related overgrowth spectrum (PROS). Clinical diagnostic criteria are also available.[1],[2] Conditions grouped under the umbrella of PROS show phenotypic variability depending on the tissue affected (mesodermal and neuroectodermal). This includes fibroadipose overgrowth (FAO), hemi hyperplasia multiple lipomatosis (HHML), congenital lipomatous overgrowth, vascular malformations, epidermal nevi, spinal and skeletal (CLOVES) syndrome, macrodactyly syndrome, the megalencephaly syndromes such as megalencephaly capillary malformation (MCAP) and megalencephaly-polymicrogyria -polydactyly-hydrocephalus (MPPH) syndromes[1] and congenital diffuse infiltrative lipomatosis (CDIL).[3] There may be entities that cannot be categorized into these phenotypes reported in varying frequencies (4–25.8%) in a few small series.[4],[5],[6] Overgrowth may be present at birth or develop postnatally and is progressive. Activating post-zygotic mutations in the PIK3CA result in disproportionate but benign overgrowth with adipose tissue dysregulation. This is noteworthy since certain solid organ cancers have been associated with similar mutations.[7] Data on the clinical profile of PROS are limited to case reports and few series, [2,4-6,8-11] though there are large multicentric studies on the implicated mutation.[2],[12]


   Subjects and Methods Top


We present a series of 15 patients with clinical features of PROS who presented to the Department of Dermatology at a tertiary care center in India from July 2012 to April 2018 (70 months) identified through a retrospective review. Ethical clearance was obtained from the Institutional Review Board. A presumptive diagnosis of PROS was made based on the diagnostic criteria proposed by Keppler- Noreuil et al. [Table 1].[1] Data were abstracted from electronic medical records and included demographic details, clinical features, and radiological imaging such as magnetic resonance imaging (MRI)/computed tomogram (CT)/ultrasonogram (USG)/X-ray. Histopathology of the overgrown tissue was available in two patients. Radiological images were retrieved from stored data images and analyzed again by a senior radiologist for the purpose of this study. Descriptive statistics such as frequency and mean were calculated. Genetic studies were not performed.
Table 1: Diagnostic criteria for PROS (Keppler-Noreuil et al.) applied to our series

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   Results Top


Demography

Fifteen patients (nine males and six females) fulfilled the criteria for a presumptive diagnosis of PROS.[1] This included FAO (n = 5), CLOVES (n = 5), MCAP (n = 2), HHML (n = 1), CDIL (n = 1) and unclassifiable (n = 1). The mean age at presentation was 12.10 years (range: 8 months to 73 years). (One of the patients with CLOVES syndrome was published as a case report previously.[13])

Clinical features

Features related to overgrowth

Overgrowth was congenital in onset in 80% (12/15) and from early childhood in the rest. It was gradually progressive in all cases and was commonly observed on the left-sided limbs or face i.e., 53.3% (8/15) vs 20% (3/15) on the right.

Overgrowth of the extremities was noted in 86.6% (13/15). The entire limb was hypertrophied in eight patients [Figure 1]a, the distal limb in five patients; three of whom had an overgrowth of only the foot/hand [Figure 1]b. The predominant pattern of limb overgrowth was of a single limb in 61.5% (8/13) with the lower limb in seven and the upper limb in one [Figure 1]c. Among the remaining five patients, three had an overgrowth of both feet, one had ipsilateral upper and lower limbs affected while the other had both upper limbs and one lower limb affected. Hemifacial hypertrophy was seen in one each of CDIL [Figure 1]d and CLOVES. Macrocephaly was noted in both patients with the MCAP syndromes and in the patient with the unclassified PROS [Table 2].
Figure 1: (a) Overgrowth of the entire right lower limb with macrodactyly of both feet and syndactyly of the left 2nd and 3rd toes in the fibroadipose overgrowth (FAO); (b) overgrowth of both feet with increased sandal gap in FAO; (c) deltoid lipoma, overgrowth of the distal one-third of the right upper limb with macrodactyly of the right thumb in FAO; (d) hemihypertrophy of the left side of face in Congenital diffuse infiltrating lipomatosis (CDIL)

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Table 2: Pattern of overgrowth in our series

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Asymmetrical truncal enlargement was seen with truncal lipomas in 46.6% (7/15) patients, that is, in all five of CLOVES syndrome [Figure 2] and in one each of FAO and HHML. Hemifacial lipoatrophy was noted in the patient with unclassified PROS.
Figure 2: Lipomas on the right mammary area and the right side of the abdominal wall in CLOVES

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Acral limb malformations

Overall, malformations of the hands or feet were found in 73.3% (11/15) which included MCAP (n = 1), CLOVES (n = 4), FAO (n = 5) and unclassified PROS (n = 1). Gross macrodactyly was noted in 40% (6/15) [Figure 1]a. Increased sandal gap was seen in 40% (6/15) patients [Figure 1]b and a similar pattern was seen in a patient with upper limb involvement who had a widened first finger web space. Other digital anomalies included syndactyly in 26.6% (4/15) [Figure 1]a, polydactyly in 13.3% (2/15), clinodactyly in 13.3% (2/15) and flexion contracture of toes in 6% (1/15) which involved the normal-appearing foot as well.

Cutaneous features

Epidermal nevi were found in 46.6% (7/15) patients which included CLOVES syndrome (n = 3) [Figure 3]a, FAO (n = 1), MCAP (n = 1), CDIL (n = 1), and the unclassified variant (n = 1). One patient with CLOVES had linear papillomatous growths on the tongue and upper lip mucosa [Figure 3]b. Epidermal nevi not only involved the overgrown limb but also extended beyond in 3/7 patients all being CLOVES. Epidermal nevus limited to the overgrowth was seen only in CDIL [Figure 3]c.
Figure 3: (a) Epidermal nevus at the nape of the neck in CLOVES; (b) linear papillomatous growths on the left side of the tongue in CLOVES; (c) epidermal nevus overlying facial overgrowth in CDIL

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Vascular malformations were found in 60% (9/15) patients; capillary malformations being the commonest, that is, 80% (8/9) [Figure 4]a and [Figure 4]b. Veno-lymphatic malformations were found in three patients, two with CLOVES syndrome and one with FAO. Two of these were present on the overgrown limb. Varicose veins of the overgrown limb were noted in two other patients. Vascular malformations involved and extended beyond the overgrowth in 5/9 patients; CLOVES (n = 3), MCAP (n = 1), and FAO (n = 1). Acrochordons were seen in 13.3% (2/15)—one adjacent to the overgrown upper limb on the anterior axillary fold [Figure 5] and in another in the gluteal cleft adjacent to the overgrown lower limb. Other cutaneous features noted were cafe au lait macules in 13.3% (2/15), hypertrichosis of the overgrown limb in 13.3% (2/15); in HHML [Figure 6]a and the FAO [Figure 6]b and blaschkoid hypopigmentation [Figure 7] which was found to be unrelated to the overgrowth in 6.6% (1/15).
Figure 4: (a) Geographic capillary malformation overlying veno-lymphatic malformation of the overgrown right lower limb in CLOVES; (b) extensive reticulate capillary malformation in Megalencephaly-capillary malformation (MCAP) syndrome

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Figure 5: Acrochordons on the anterior axillary fold adjacent the overgrown right upper limb in FAO

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Figure 6: Hypertrichosis of the overgrown left lower limb in (a) hemi hyperplasia multiple lipomatosis (HHML); (b) in FAO

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Figure 7: Blaschkoid hypopigmentation on the neck in FAO

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Skeletal involvement

One patient with CLOVES had scoliosis, recurrent patellar subluxation, bilateral genu recurvatum, and hyper-extensible joints.

Neurological involvement

The central nervous system was affected in 26.6% (4/15) which included both the MCAP and one each of CLOVES and FAO. There was a history of developmental delay in three patients, one each with MCAP, CLOVES, and FAO, seizures in one patient each with MCAP and FAO. Posterior spina bifida was also present in the patient with FAO.

Genital abnormalities

Among the nine male patients, genital abnormalities were found in 33.3% (3/9); FAO (n = 2) and CLOVES (n = 1). These included cryptorchidism in two patients, hydrocoele and hypospadias in one patient each. In the two female patients in whom imaging was available no ovarian abnormalities were found.

Ocular abnormalities

One of the patients with MCAP syndrome was detected to have congenital glaucoma and corneal clouding.

Dentition

Premature dentition with macrodontia was seen in the child with CDIL.

The clinical features of individual patients are summarized in [Table 3].
Table 3: Demographic and clinical features of individual patients (n=15)

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Investigations

Radiological imaging of the hypertrophied extremity was performed in 60% (9/15) patients in whom the predominant lipomatous nature of the overgrowth was ascertained. Additionally, muscular and osseous hypertrophy was noted in 3/9, osseous hypertrophy alone in 4/9 [Figure 8]a. MRI revealed fatty infiltration of the underlying musculature in three patients [Figure 8]b. In one patient with FAO, the discrete truncal lipoma was also found to infiltrate the underlying musculature.
Figure 8: (a) Soft tissue and bony hypertrophy of the left foot with macrodactyly, increased sandal gap on X-ray in MCAP; (b) MRI T1 coronal images showing left lower limb hemi hyperplasia with increased unencapsulated fat in subcutaneous plane (asterix), intermuscular fascial planes (black arrows), and intramuscular plane (white arrows) in HHML; (c) MRI brain T2W showing left hemimegalencephaly in MCAP

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MRI of the brain was done for four patients with neurological symptoms. Hemimegalencephaly was detected in three patients; MCAP (n = 1) [Figure 8]c, CLOVES (n = 1), and FAO (n = 1). The one other patient with MCAP had cerebral atrophy.

Visceral imaging including USG or MRI, or CT scan were available in nine patients which detected complicated pararenal cyst, small malrotated kidney and parietal peritoneal cysts in one patient each.

Histopathology of epidermal nevi done for two patients and of acrochordons done for one patient showed features consistent with the clinical diagnosis.


   Discussion Top


PROS is a sporadic overgrowth syndrome with unknown prevalence. The estimated incidence of CLOVES is less than 1:1,000,000 with less than 200 reported cases.[14] Around 70 cases of CDIL have been reported[15] and more than 150 cases of MCAP have been reported. There is no clear data on the prevalence of the less defined FAO and the rarer HHML. These disorders have been reported across various ethnicities and do not have any gender predilection; the largest series of PROS reports a male: female ratio of 1:1.3. Keppler-Noreuil et al. and Kuentz et al. proposed clinical diagnostic and testing eligibility criteria for PROS.[1],[2] All patients included in our study fulfilled the clinical criteria for a presumptive diagnosis of PROS.[1] It could not, however, be confirmed with mutation analysis.

In our case series of 15 patients with PROS, all except one patient could be assigned a phenotypic diagnosis. Among our patients, CLOVES and FAO were the most common subtypes, seen in five patients each. Keppler et al. had reported a frequency of 45.7% of FAO and 25.7% of CLOVES in their series.[9] Distal limb malformations were found in all series described thus far.[4],[6],[8],[9],[10],[11] The largest series reported a frequency of 22.8% (8/35)[9] whereas our series had 73.3% (11/15). The infiltrative nature of the fatty overgrowth was also noted in some of our patients as has been documented.[16]

The description of cutaneous features was sparse across the literature. Epidermal nevi, seborrheic keratosis, and lichenoid keratosis are the cutaneous features listed under PROS.[1] We report a higher frequency of epidermal nevi as compared to the largest descriptive series reported in literature (46.6% vs 11.4% in Keppler-Noreuil et al.'s series) and a higher frequency of vascular malformations as well (60% vs 57.1% in Hucthagowder et al.'s series and 42% in Keppler-Noreuil et al.'s series). Capillary malformations were the commonest type of vascular malformation as noted in previous studies.[9] These cutaneous features were not limited to the overgrown tissues but extended beyond and sometimes were unrelated to the overgrowth. Epidermal nevi have been found overlying the lipomatous overgrowth in CLOVES.[17] The unusual findings in our series were acrochordons adjacent to the overgrown extremity in two patients with FAO, blaschkoid hypopigmentation in one patient with FAO and linear papillomatous growths in the oral mucosa on the same side as the hemifacial hypertrophy in one patient with CLOVES. Focal or segmental acrochordons have not been reported in association with PROS whereas multiple acrochordons occur in inherited conditions such as Birt-Hogg-Dube syndrome, tuberous sclerosis, and PTEN hamartoma syndrome.

Regional lipoatrophy was noted in 25.7% (9/35) patients by Keppler-Noreuil et al. We had one patient with hemifacial atrophy. Other reported cutaneous features such as hypotrichosis, dermal melanocytosis, and haemangiomas were not seen in our series.

Neurological involvement in CLOVES syndrome is a relatively rare occurrence[18] which we found in one of our patients in the form of delayed developmental milestones and megalencephaly. Of the two patients with MCAP, one had typical features whereas the other had features that overlapped with CLOVES syndromes such as the presence of epidermal nevus and distal limb malformations with overgrowth This overlap has been termed as MCAP-plus in literature.[17] The patients with CDIL, HHML were not found to have any unusual features than those reported in the literature.

Visceral abnormalities in PROS involve mesodermal structures such as the renal and the reproductive system. An abnormal PIK3/mTor/AKT1 pathway is responsible for cellular proliferation forming renal cysts in polycystic kidney disease. Whether the same defect is responsible for the increased frequency of renal and genital abnormalities in the absence of involvement of other mesodermal structures needs to be further explored and is limited by the difficulty in obtaining the necessary tissue to analyze mutations.[19] Renal abnormalities were noted only in one of our patients (11.1%, 1/9) whereas Keppler et al. had observed the same in 42% (11/26) of patients in whom it was assessed.[9] Sapp et al. and Alomari et al. have made similar observations in their series of CLOVES.[16],[20] Male genital abnormalities have been reported by Keppler-Noreuil et al. with a frequency of 26.6% (4/15)[9] and by Sapp et al. in two patients in whom it was looked for in the original series of CLOVES.[14] Male genital abnormalities were noted at a higher frequency of 33.3% (3/9) in our study.

A functioning PI3K-AKT-mTOR pathway is required for the development of vascular, limb and brain structures[21] which is also regulated by extrinsic mechanisms.[22] To date, 41 variants of PIK3CA mutations have been reported in PROS.[12] Many authors surmise that the phenotypic variation in PROS may be dependent on the timing and the coding sequence affected during embryogenesis.[8],[23] Diagnostic molecular studies are hampered by several factors including the best tissue to sample, prevalent low levels of mosaicism (<5%) and the low sensitivity and specificity of conventional techniques. Mutations have been identified in lesional skin fibroblasts, keratinocytes, fatty tissue, neural elements, muscles, bone, vascular malformations, brain hemispherectomy samples[9] and even a few saliva and peripheral blood lymphocyte samples in MCAP,[24] often requiring tissue cultures. Highly sensitive techniques such as targeted deep sequencing of genes coupled with high depth next-generation sequencing, droplet digital polymerase chain reaction, restriction fragment length polymorphism (RFLP) and others are preferred over Sanger sequencing and standard depth next-generation sequencing.[5],[9] Targeted deep sequencing methods have reported a diagnostic yield of up to 71%.[5]

The current treatment strategy for PROS is primarily medical using m-Tor inhibitors whose optimum therapeutic potential remains to be ascertained over time.[22] Lipomatous overgrowth seems to relapse despite liposuction and surgical debulking is often complicated by lipomatous infiltration into the underlying tissues.[10] Seizures associated with hemimegalencephaly have required hemispherectomy of the affected side in addition to medical management in some cases.[25] Veno-lymphatic malformations in these syndromes may be managed with sclerotherapy following or prior to medical therapy. Alpelisib (BYL719), a specific PIK3CA inhibitor that is currently undergoing clinical trials holds promise with successful short term outcomes to date.[26]

Limitation

Mutation analysis was not performed to substantiate the diagnosis.


   Conclusions Top


Our case series has clinical features in keeping with the PROS with few unreported mucocutaneous features such as acrochordons, blaschkoid hypopigmentation, and linear papillomatous growth in the oral mucosa. Atypical clinical features found in certain phenotypes and the overlap of features between the various phenotypes in PROS as noted in our series suggest that a genotypic categorization would be superior to phenotypic categorization. However, in resource-poor settings, clinical criteria may be adequate for the diagnosis of these mosaic conditions considering the limited accessibility of expensive molecular tests to detect mutations that are present only at low levels. Besides being a functionally and socially debilitating condition, identifying PROS is important so that patients are screened for genitourinary complications and for tumor surveillance. In addition, there is a possibility of treatment based on emerging data on the development of specific PIK3CA inhibitors.[26] A multidisciplinary team of specialists is required for accurate clinical evaluation and optimum management. The clinical features of this series of patients from the Indian subcontinent add to the phenotypic profile of PROS.

Declaration of patient consent

The authors certify that they have obtained all appropriate patient consent forms. In the form the patient(s) has/have given his/her/their consent for his/her/their images and other clinical information to be reported in the journal. The patients understand that their names and initials will not be published and due efforts will be made to conceal their identity, but anonymity cannot be guaranteed.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Keppler-Noreuil KM, Rios JJ, Parker VE, Semple RK, Lindhorst MJ, Sapp JC, et al. PIK3CA-related overgrowth spectrum (PROS): Diagnostic and testing eligibility criteria, differential diagnosis, and evaluation. Am J Med Genet A 2015;167A: 287-95.  Back to cited text no. 1
    
2.
Kuentz P, St-Onge J, Duffourd Y, Courcet JB, Carmignac V, Jouan T, et al. Molecular diagnosis of PIK3CA-related overgrowth spectrum (PROS) in 162 patients and recommendations for genetic testing. Genet Med 2017;19:989-97.  Back to cited text no. 2
    
3.
Maclellan RA, Luks VL, Vivero MP, Mulliken JB, Zurakowski D, Padwa BL, et al. PIK3CA activating mutations in facial infiltrating lipomatosis. Plast Reconstr Surg 2014;133:12e-9e.  Back to cited text no. 3
    
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Yeung KS, Ip JJ, Chow CP, Kuong EY, Tam PK, Chan GC, et al. Somatic PIK3CA mutations in seven patients with PIK3CA-related overgrowth spectrum. Am J Med Genet A 2017;173:978-84.  Back to cited text no. 4
    
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Hucthagowder V, Shenoy A, Corliss M, Vigh-Conrad KA, Storer C, Grange DK, et al. Utility of clinical high-depth next generation sequencing for somatic variant detection in the PIK3CA-related overgrowth spectrum. Clin Genet 2017;91:79-85.  Back to cited text no. 5
    
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Vahidnezhad H, Youssefian L, Baghdadi T, Sotoudeh S, Tavassoli A, Zeinali S, et al. Phenotypic heterogeneity in PIK3CA-related overgrowth spectrum. Br J Dermatol 2016;175:810-4.  Back to cited text no. 6
    
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Madsen RR, Vanhaesebroeck B, Semple RK. Cancer-associated PIK3CA mutations in overgrowth disorders. Trends Mol Med 2018;24:856-70.  Back to cited text no. 7
    
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Loconte DC, Grossi V, Bozzao C, Forte G, Bagnulo R, Stella A, et al. Molecular and functional characterization of three different postzygotic mutations in PIK3CA-related overgrowth spectrum (PROS) patients: Effects on PI3K/AKT/mTOR signaling and sensitivity to PIK3 inhibitors. PLoS One 2015;10:e0123092.  Back to cited text no. 8
    
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Keppler-Noreuil KM, Sapp JC, Lindhurst MJ, Parker VE, Blumhorst C, Darling T, et al. Clinical delineation and natural history of the PIK3CA-related overgrowth spectrum. Am J Med Genet A 2014;164A:1713-33.  Back to cited text no. 9
    
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Rasmussen M, Sunde L, Weigert KP, Bogaard PW, Lildballe DL. Segmental overgrowth syndrome due to an activating PIK3CA mutation identified in affected muscle tissue by exome sequencing. Am J Med Genet A 2014;164A:1318-21.  Back to cited text no. 10
    
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Rios JJ, Paria N, Burns DK, Israel BA, Cornelia R, Wise CA, et al. Somatic gain-of-function mutations in PIK3CA in patients with macrodactyly. Hum Mol Genet 2013;22:444-51.  Back to cited text no. 11
    
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Mirzaa G, Timms AE, Conti V, Boyle EA, Girisha KM, Martin B, et al. PIK3CA-associated developmental disorders exhibit distinct classes of mutations with variable expression and tissue distribution. JCI Insight 2016;1:e87623.  Back to cited text no. 12
    
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Gopal B, Keshava SN, Selvaraj D. A rare newly described overgrowth syndrome with vascular malformations-cloves syndrome. Indian J Radiol Imaging 2015;25:71-3.  Back to cited text no. 13
[PUBMED]  [Full text]  
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Alomar S, Khedr RE, Alajlan S. CLOVES syndrome in a nine-month-old infant. Cureus 2019;11:e5772.  Back to cited text no. 14
    
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Sun R, Sun L, Li G, Sun Z, Zhao Y, Ma X, et al. Congenital infiltrating lipomatosis of the face: A subtype of hemifacial hyperplasia. Int J Pediatr Otorhinolaryngol 2019;125:107-12.  Back to cited text no. 15
    
16.
Sapp JC, Turner JT, van de Kamp JM, van Dijk FS, Lowry RB, Biesecker LG. Newly delineated syndrome of congenital lipomatous overgrowth, vascular malformations, and epidermal nevi (CLOVE syndrome) in seven patients. Am J Med Genet A 2007;143A:2944-58.  Back to cited text no. 16
    
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Mirzaa G, Conway R, Graham JM, Dobyns WB. PIK3CA-related segmental overgrowth. In: Adam MP, Ardinger HH, Pagon RA, Wallace SE, Bean LJ, Stephens K, et al. editors. GeneReviews®. Seattle (WA): University of Washington, Seattle; 1993.  Back to cited text no. 17
    
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Martinez-Lopez A, Blasco-Morente G, Perez-Lopez I, Herrera-Garcia JD, Luque-Valenzuela M, Sanchez-Cano D, et al. CLOVES syndrome: Review of a PIK3CA-related overgrowth spectrum (PROS). Clin Genet 2017;91:14-21.  Back to cited text no. 18
    
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De Santis MC, Sala V, Martini M, Ferrero GB, Hirsch E. PI3K signaling in tissue hyper-proliferation: From overgrowth syndromes to kidney cysts. Cancers 2017;9:30.  Back to cited text no. 19
    
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Alomari AI. Characterization of a distinct syndrome that associates complex truncal overgrowth, vascular, and acral anomalies: A descriptive study of 18 cases of CLOVES syndrome. Clin Dysmorphol 2009;18:1-7.  Back to cited text no. 20
    
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Barrett D, Brown VI, Grupp SA, Teachey DT. Targeting the PI3K/AKT/mTOR signaling axis in children with hematologic malignancies. Paediatr Drugs 2012;14:299-316.  Back to cited text no. 22
    
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Barker KT, Houlston RS. Overgrowth syndromes: Is dysfunctional PI3-kinase signalling a unifying mechanism? Eur J Hum Genet 2003;11:665-70.  Back to cited text no. 23
    
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Rivière JB, Mirzaa GM, O'Roak BJ, Beddaoui M, Alcantara D, Conway RL, et al. De novo germline and postzygotic mutations in AKT3, PIK3R2 and PIK3CA cause a spectrum of related megalencephaly syndromes. Nat Genet 2012;44:934-40.  Back to cited text no. 24
    
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Lee JH, Huynh M, Silhavy JL, Kim S, Dixon-Salazar T, Heiberg A, et al. De novo somatic mutations in components of the PI3K-AKT3-mTOR pathway cause hemimegalencephaly. Nat Genet 2012;44:941-5.  Back to cited text no. 25
    
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Venot Q, Blanc T, Rabia SH, Berteloot L, Ladraa S, Duong JP, et al. Targeted therapy in patients with PIK3CA-related overgrowth syndrome. Nature 2018;558:540-6.  Back to cited text no. 26
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8]
 
 
    Tables

  [Table 1], [Table 2], [Table 3]



 

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